Author Correction: An in planta biolistic method for stable wheat transformation
نویسندگان
چکیده
منابع مشابه
In-planta transformation: recent advances
In-planta transformation is an efficient, quick and tissue culture independent system for crop plants improvement. In-planta transformation is a useful system for those plants that lack tissue culture and regeneration system. Therefore, the direct in-planta methods are commonly used for the transformation of many important genes into these plant species. The two most common Agrobacterium mediat...
متن کاملBiolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers.
Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella with the linear 1Dx5 gene without selectable markers. The highest tra...
متن کاملEffects of Ammonium Nitrate upon Direct Somatic Embryogenesis and Biolistic Transformation of Wheat
Triticum aestivum is of major importance both nutritionally and economically globally. Traditional breeding mechanisms have been unsuccessful at keeping pace with the increasing demand for better yielding and more resilient wheat varieties. The introduction of foreign genes into systems has provided a new tool for crop improvement, but has been difficult to apply to elite wheat varieties mainly...
متن کاملVariant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat
Genetic transformation via the biolistic method has been used to introduce genes encoding natural and novel high-molecular-weight glutenin subunits (HMW-GS) into wheat. The appearance of new seed proteins of sizes not predicted by the transgene coding sequences was noted in some experiments. In this report, the identities of thirteen of these novel proteins were determined by tandem mass spectr...
متن کاملBiolistic transformation of a procaryote, Bacillus megaterium.
We present a simple and rapid method for introducing exogenous DNA into a bacterium, Bacillus megaterium, utilizing the recently developed biolistic process. A suspension of B. megaterium was spread onto the surface of nonselective medium. Plasmid pUB110 DNA, which contains a gene that confers kanamycin resistance, was precipitated onto tungsten particles. Using a biolistic propulsion system, t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-17188-2